Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells
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چکیده
منابع مشابه
Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells
Antibodies are valuable tools for functional studies in vitro, but their use in living cells remains challenging because they do not naturally cross the cell membrane. Here, we present a simple and highly efficient method for the intracytoplasmic delivery of any antibody into cultured cells. By following the fate of monoclonal antibodies that bind to nuclear antigens, it was possible to image e...
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ژورنال
عنوان ژورنال: mAbs
سال: 2013
ISSN: 1942-0862,1942-0870
DOI: 10.4161/mabs.25084